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633 (1:200) and nuclear counterstain 4,6-diamidino-2phenylindole (DAPI, 0.1 g/ml; Molecular Probes). Secondary only control wells (including DAPI) were included for every time point and/or sorted subpopulation. Stained wells were mounted in 75 glycerol in PBS. For EdU experiments, we used the Click-iT EdU Alexa Fluor 488 HCS Assay (Molecular Probes) prior to primary antibody addition. Cells were
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633 (1:200) and nuclear counterstain 4,6-diamidino-2phenylindole (DAPI, 0.1 g/ml; Molecular Probes). Secondary only control wells (including DAPI) were included for every time point and/or sorted subpopulation. Stained wells were mounted in 75 glycerol in PBS. For EdU experiments, we used the Click-iT EdU Alexa Fluor 488 HCS Assay (Molecular Probes) prior to primary antibody addition. Cells were
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Nt in the red (red line) and the black (black line) cues. In the starting, behaviour is absolutely goal-directed in each contexts. Contingencies are unknown and hence actions are selected randomly, top generally to shock and therefore to a far more damaging Pavlovian worth. Having said that, in the similar time understanding about stimulus-action-outcomevalue associations improves with finding out
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Under one village, they preferred to list not only the village but also the sub area to help them identify the children. Internet access in some remote areas could be unstable and the system upgrade sometimes encountered problems. These factors also compromised usage of EPI app by village doctors. Only one village doctor reported that it was very hard for him to learn how to operate the EPI app an
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E data: CGT. Wrote the manuscript: RHuang, XL, RHolm, JMN, CGT, ZS. All authors read and approved the final manuscript. All authors read and approved the final manuscript. Acknowledgments This study was financially supported by grants from Inger and John Fredriksen Foundation and Radium Hospital Research Foundation and The Norwegian Cancer Society. We thank Ellen Hellesylt, Mette Synn e F sund, Ma