Each and every reaction was executed in a final volume of 25 l made up of just one l cDNA and a couple of x SYBR Eco-friendly PCR Learn blend. The amplification profile was denaturation at ninety four for 2 min, followed by forty cycles of 94 for ten sec, fifty eight for 30 sec. Within the close in the PCR cycles, melting curve assessment of your PCR goods was carried out to validate the amplif